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Nat Methods. 2009 Jan;6(1):79-81. doi: 10.1038/nmeth.1278. Epub 2008 Dec 14.

Infrared laser-mediated gene induction in targeted single cells in vivo.

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1
Radiation Biology Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto, Japan. ykamei@radbio.med.osaka-u.ac.jp

Abstract

We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.

PMID:
19079252
DOI:
10.1038/nmeth.1278
[Indexed for MEDLINE]
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