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EMBO J. 2009 Jan 21;28(2):123-34. doi: 10.1038/emboj.2008.262. Epub 2008 Dec 11.

Circadian gene expression is resilient to large fluctuations in overall transcription rates.

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1
Department of Molecular Biology & NCCR Frontiers in Genetics, University of Geneva, Geneva, Switzerland.

Abstract

Mammalian circadian oscillators are considered to rely on transcription/translation feedback loops in clock gene expression. The major and essential loop involves the autorepression of cryptochrome (Cry1, Cry2) and period (Per1, Per2) genes. The rhythm-generating circuitry is functional in most cell types, including cultured fibroblasts. Using this system, we show that significant reduction in RNA polymerase II-dependent transcription did not abolish circadian oscillations, but surprisingly accelerated them. A similar period shortening was observed at reduced incubation temperatures in wild-type mouse fibroblasts, but not in cells lacking Per1. Our data suggest that mammalian circadian oscillators are resilient to large fluctuations in general transcription rates and temperature, and that PER1 has an important function in transcription and temperature compensation.

PMID:
19078963
PMCID:
PMC2634731
DOI:
10.1038/emboj.2008.262
[Indexed for MEDLINE]
Free PMC Article
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