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EMBO J. 2009 Feb 4;28(3):193-204. doi: 10.1038/emboj.2008.264. Epub 2008 Dec 11.

RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E. coli.

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  • 1Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.

Abstract

The bacterial MreB actin cytoskeleton is required for cell shape maintenance in most non-spherical organisms. In rod-shaped cells such as Escherichia coli, it typically assembles along the long axis in a spiral-like configuration just underneath the cytoplasmic membrane. How this configuration is controlled and how it helps dictate cell shape is unclear. In a new genetic screen for cell shape mutants, we identified RodZ (YfgA) as an important transmembrane component of the cytoskeleton. Loss of RodZ leads to misassembly of MreB into non-spiral structures, and a consequent loss of cell shape. A juxta-membrane domain of RodZ is essential to maintain rod shape, whereas other domains on either side of the membrane have critical, but partially redundant, functions. Though one of these domains resembles a DNA-binding motif, our evidence indicates that it is primarily responsible for association of RodZ with the cytoskeleton.

PMID:
19078962
PMCID:
PMC2637328
DOI:
10.1038/emboj.2008.264
[PubMed - indexed for MEDLINE]
Free PMC Article
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