Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20637-40. doi: 10.1073/pnas.0810822105. Epub 2008 Dec 12.

Multiplex protein assays based on real-time magnetic nanotag sensing.

Author information

1
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.

Abstract

Magnetic nanotags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, because minute quantities of MNTs can be detected with inexpensive giant magnetoresistive (GMR) sensors, such as spin valve (SV) sensors. However, translating this promise into easy to use and multilplexed protein assays, which are highly sought after in molecular diagnostics such as cancer diagnosis and treatment monitoring, has been challenging. Here, we demonstrate multiplex protein detection of potential cancer markers at subpicomolar concentration levels and with a dynamic range of more than four decades. With the addition of nanotag amplification, the analytic sensitivity extends into the low fM concentration range. The multianalyte ability, sensitivity, scalability, and ease of use of the MNT-based protein assay technology make it a strong contender for versatile and portable molecular diagnostics in both research and clinical settings.

PMID:
19074273
PMCID:
PMC2602607
DOI:
10.1073/pnas.0810822105
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center