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Head Neck. 2009 Mar;31(3):371-80. doi: 10.1002/hed.20968.

Tumor-derived microvesicles in sera of patients with head and neck cancer and their role in tumor progression.

Author information

1
Departments of Pathology and Otolaryngology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, USA.

Abstract

BACKGROUND:

Tumor-derived membranous vesicles (MV) isolated from sera of the patients with squamous cell carcinomas of the head and neck (HNSCC) induce apoptosis of activated CD8(+) T cells. We tested if MV molecular profile and activity correlate with disease progression.

METHODS:

CD8(+) Jurkat cells were incubated with MAGE 3/6(+), FasL(+), MHC class I(+) MV isolated from sera of 60 patients with HNSCC and 25 normal controls by exclusion chromatography and ultracentrifugation. Z-VAD-FITC binding to Jurkat was measured and correlated with clinical data.

RESULTS:

MV from patients' sera, but not from sera of normal controls, induced Jurkat cell apoptosis. Forty-five percent T cells+MV from patients with N(1)-N(3) disease were apoptotic versus 18% T cells+MV from patients with N(0) disease (p < .008). MV from patients with active disease (AD) expressed higher FasL levels than MV from patients with no evident disease (NED) or normal controls (p <or= .01).

CONCLUSION:

MAGE 3/6(+), FasL(+), and MHCI(+) MV in sera of patients induced T-cell apoptosis, which correlated with disease activity and the presence of lymph node metastases.

PMID:
19073006
PMCID:
PMC2647573
DOI:
10.1002/hed.20968
[Indexed for MEDLINE]
Free PMC Article

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