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J Proteome Res. 2009 Feb;8(2):443-9. doi: 10.1021/pr800645r.

ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.

Author information

1
Department of Chemistry, University of Toronto, Toronto, Ontario, M5S 3H6, Canada.

Abstract

Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, and may be readily elaborated to many different assay formats.

PMID:
19072657
PMCID:
PMC2646003
DOI:
10.1021/pr800645r
[Indexed for MEDLINE]
Free PMC Article

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