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Talanta. 2007 Feb 15;71(2):668-75. doi: 10.1016/j.talanta.2006.05.014. Epub 2006 Jun 13.

Simultaneous analysis and identification of main bioactive constituents in extract of Zizyphus jujuba var. sapinosa (Zizyphi spinosi semen) by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry.

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1
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, ChangSha 410081, PR China.

Abstract

A high-performance liquid chromatography (HPLC) coupled with UV photodiode array detection and electrospray mass spectrometry (HPLC-DAD-ESI-MS) was developed for the simultaneous analysis and identification of main bioactive constituents in extract of zizyphi spinosi semen including three flavonoids, three alkaloids and four triterpene saponins. The separation was achieved on a C(18) column with a gradient solvent system of 0.08% aqueous formic acid solution-acetonitrile. The compounds were identified with MS-spectra and authentic standards. The contents of jujuboside A, jujuboside B, spinosin and 6'''-feruloyl spinosin were quantified by using DAD in different extract of zizyphi spinosi semen.

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