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Anal Bioanal Chem. 2009 Feb;393(4):1135-41. doi: 10.1007/s00216-008-2521-y. Epub 2008 Dec 7.

SERS-based plasmonic nanobiosensing in single living cells.

Author information

1
Department of Biomedical Engineering, Duke University, 136 Hudson Hall, P. O. Box 90281, Durham, NC 27708, USA.

Abstract

In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level.

PMID:
19066865
PMCID:
PMC4022298
DOI:
10.1007/s00216-008-2521-y
[Indexed for MEDLINE]
Free PMC Article

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