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J Vis Exp. 2008 Jul 8;(17). pii: 760. doi: 10.3791/760.

Staining proteins in gels.

Author information

1
Keck Graduate Institute of Applied Life Sciences, UVP, LLC, USA.

Abstract

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit describes protocols for detecting proteins by four popular methods. Coomassie blue staining is an easy and rapid method. Silver staining, while more time consuming, is considerably more sensitive and can thus be used to detect smaller amounts of protein. Fluorescent staining is a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and is often as sensitive as silver staining. Staining of proteins with SYPRO Orange and SYPRO Ruby are also demonstrated here.

PMID:
19066521
PMCID:
PMC3253607
DOI:
10.3791/760
[Indexed for MEDLINE]
Free PMC Article

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