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Opt Express. 2008 Dec 8;16(25):20742-7.

Ultrafast time-resolved fluorescence by two photon absorption excitation.

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Department of Chemistry, Pohang University of Science and Technology, Pohang, S. Korea.


We report a time-resolved fluorescence apparatus utilizing fluorescence upconversion by noncollinear sum frequency generation and two photon absorption as an excitation. Near perfect time-resolution is achieved with 20 fs pulses to give the instrument response of 33 fs (FWHM) over the entire fluorescence wavelength for a 100 microm thick mixing crystal. Through experiments and numerical simulations, it is shown that 40 fs time-resolution can be obtained even for a 580 microm thick mixing crystal at a fluorescence wavelength longer than 500 nm.

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