Format

Send to

Choose Destination
Cell Host Microbe. 2008 Dec 11;4(6):592-9. doi: 10.1016/j.chom.2008.10.013.

Three-dimensional analysis of budding sites and released virus suggests a revised model for HIV-1 morphogenesis.

Author information

1
Abteilung Virologie, Universitätsklinikum D-69120 Heidelberg, Germany.

Abstract

Current models of HIV-1 morphogenesis hold that newly synthesized viral Gag polyproteins traffic to and assemble at the cell membrane into spherical protein shells. The resulting late-budding structure is thought to be released by the cellular ESCRT machinery severing the membrane tether connecting it to the producer cell. Using electron tomography and scanning transmission electron microscopy, we find that virions have a morphology and composition distinct from late-budding sites. Gag is arranged as a continuous but incomplete sphere in the released virion. In contrast, late-budding sites lacking functional ESCRT exhibited a nearly closed Gag sphere. The results lead us to propose that budding is initiated by Gag assembly, but is completed in an ESCRT-dependent manner before the Gag sphere is complete. This suggests that ESCRT functions early in HIV-1 release--akin to its role in vesicle formation--and is not restricted to severing the thin membrane tether.

PMID:
19064259
PMCID:
PMC3454483
DOI:
10.1016/j.chom.2008.10.013
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center