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J Mol Biol. 2009 Jan 30;385(4):1165-78. doi: 10.1016/j.jmb.2008.11.024. Epub 2008 Nov 25.

The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases.

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1
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovského nám. 2, 162 06 Praha 6, Czech Republic. skalova@imc.cas.cz

Abstract

The X-ray structure of the two-domain laccase (small laccase) from Streptomyces coelicolor A3(2) was solved at 2.7-A resolution. The enzyme differs significantly from all laccases studied structurally so far. It consists of two domains and forms trimers and hence resembles the quaternary structure of nitrite reductases or ceruloplasmins more than that of large laccases. There are three trinuclear copper clusters in the enzyme localized between domains 1 and 2 of each pair of neighbor chains. In this way, a similar geometry of the active site as seen in large laccases is ensured, albeit by different arrangements of domains and protein chains. Three copper ions of type 1 lie close to one another near the surface of the central part of the trimer, and, effectively, a trimeric substrate binding site is formed in their vicinity.

PMID:
19063896
DOI:
10.1016/j.jmb.2008.11.024
[Indexed for MEDLINE]
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