Format

Send to

Choose Destination
J Biol Rhythms. 2008 Dec;23(6):483-8. doi: 10.1177/0748730408326682.

Increased coherence of circadian rhythms in mature fibroblast cultures.

Author information

1
Centre for Systems Biology at Edinburgh, Mayfield Road, Edinburgh, UK. john.oneill@ed.ac.uk

Abstract

Circadian transcriptional rhythms in confluent NIH 3T3 and PER2::LUCIFERASE fibroblast cultures become more coherent as they mature. This enhanced coherence correlated with increased culture density, while inhibition of the cell cycle increased coherence in immature cultures. Mature cultures may therefore express more coherent rhythms for longer because of their reduced rate of cell division. The authors conclude that mature fibroblast cultures will be of great utility for high-throughput pharmacological and mutational circadian screening.

PMID:
19060257
PMCID:
PMC2735814
DOI:
10.1177/0748730408326682
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center