Format

Send to

Choose Destination
Traffic. 2009 Feb;10(2):131-6. doi: 10.1111/j.1600-0854.2008.00859.x. Epub 2008 Nov 20.

A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos.

Author information

1
Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Brisbane, Australia.

Abstract

The zebrafish is a powerful vertebrate system for cell and developmental studies. In this study, we have optimized methods for fast freezing and processing of zebrafish embryos for electron microscopy (EM). We show that in the absence of primary chemical fixation, excellent ultrastructure, preservation of green fluorescent protein (GFP) fluorescence, immunogold labelling and electron tomography can be obtained using a single technique involving high-pressure freezing and embedding in Lowicryl resins at low temperature. As well as being an important new tool for zebrafish research, the maintenance of GFP fluorescence after fast freezing, freeze substitution and resin embedding will be of general use for correlative light and EM of biological samples.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center