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J Vet Sci. 2008 Dec;9(4):421-3.

Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs.

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1
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato, University, Towada, Aomori 034-8628, Japan.

Abstract

The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment was started. Salivary chromogranin A concentrations were measured using an enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary chromogranin A secretion, and no differences in salivary chromogranin A concentrations measured every 4 h were demonstrated during the 24-h cycle in dogs.

PMID:
19052500
PMCID:
PMC2811785
[Indexed for MEDLINE]
Free PMC Article
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