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Int J Colorectal Dis. 2009 Feb;24(2):129-38. doi: 10.1007/s00384-008-0608-8. Epub 2008 Dec 3.

Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study.

Author information

1
Department of Radiation Oncology, University Hospital Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium. Annelies.debucquoy@med.kuleuven.be

Abstract

AIMS:

The aims of the study were to study the effect of pre-operative treatment on the expression of tumour-related proteins and to correlate the expression of these proteins with response and survival of patients with advanced rectal cancer.

MATERIALS AND METHODS:

Tissue micro-arrays from pre- and post-treatment biopsies of 99 patients with rectal cancer treated with pre-operative (chemo)radiotherapy were stained for epidermal growth factor receptor (EGFR), carbonic anhydrase IX, Ki67, vascular endothelial growth factor, cyclo-oxygenase 2 (COX-2) and cleaved cytokeratin 18 (c-CK18). Also, fibro-inflammatory alterations after treatment were evaluated.

RESULTS:

Pre-operative (chemo)radiotherapy caused fibro-inflammatory changes, a downregulation of proliferation (Ki67) and EGFR and an upregulation of apoptosis (cleaved CK18). Patients with a good regression during pre-operative treatment showed less proliferating and apoptotic cells in the resection specimen. Multivariate analysis showed that T downstaging, fibro-inflammatory changes in the resection specimen and COX-2 expression in the biopsy correlated with overall survival.

CONCLUSIONS:

Pre-operative treatment has an effect on proliferation, apoptosis, inflammation and EGFR expression. The classical clinical parameters as well as fibro-inflammatory changes and COX-2 expression seem most valuable as predictors for survival.

PMID:
19050903
PMCID:
PMC2745734
DOI:
10.1007/s00384-008-0608-8
[Indexed for MEDLINE]
Free PMC Article
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