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Appl Biochem Biotechnol. 2009 May;153(1-3):139-50. doi: 10.1007/s12010-008-8433-7. Epub 2008 Dec 3.

Lime pretreatment of sugarcane bagasse for bioethanol production.

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School of Chemical Engineering, University of Campinas, UNICAMP, Campinas, Brazil.


The pretreatment of sugarcane bagasse with lime (calcium hydroxide) is evaluated. The effect of lime pretreatment on digestibility was studied through analyses using central composite design (response surface), considering pretreatment time, temperature, and lime loading as factors. The responses evaluated were the yield of glucose from pretreated bagasse after enzymatic hydrolysis. Experiments were performed using the bagasse as it comes from an alcohol/sugar factory (non-screened bagasse) and bagasse in the size range from 0.248 to 1.397 mm (screened bagasse) (12-60 mesh). It was observed that the particle size presented influence in the release of fermentable sugars after enzymatic hydrolysis using low loading of cellulase and beta-glucosidase (3.5 FPU/g dry pretreated biomass and 1.0 IU/g dry pretreated biomass, respectively).

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