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Drug Discov Today. 2009 Jan;14(1-2):102-7. doi: 10.1016/j.drudis.2008.11.006. Epub 2008 Dec 26.

3D cell culture opens new dimensions in cell-based assays.

Author information

1
Global Cell Solutions, Inc., 770 Harris Street, Suite 104, Charlottesville, VA 22903, USA. brad.justice@globalcellsolutions.com

Abstract

3D cell culture technologies have revolutionized our understanding of cellular behavior, both in culture and in vivo, but adoption by cell-based screening groups has been slow owing to problems of consistency, scale and cost. The evolving field of high content screening technologies will, however, require a rethinking of 3D cell culture adoption to ensure the next generation of cells provide relevant in vivo-like data. Three current technologies are presented in this review: membranes, sponges/gels and microcarriers. A short history of these technologies and unique research applications are discussed. Also, the technologies are evaluated for usefulness in modern automated cell-based screening equipment.

PMID:
19049902
DOI:
10.1016/j.drudis.2008.11.006
[Indexed for MEDLINE]

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