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J Microbiol Methods. 2009 Mar;76(3):305-6. doi: 10.1016/j.mimet.2008.11.001. Epub 2008 Nov 14.

An enzyme cocktail for efficient protoplast formation in Aspergillus niger.

Author information

1
Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Abstract

Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia.

PMID:
19041907
DOI:
10.1016/j.mimet.2008.11.001
[Indexed for MEDLINE]

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