PMID- 19040474
OWN - NLM
STAT- MEDLINE
DCOM- 20090102
LR  - 20081201
IS  - 1469-0691 (Electronic)
IS  - 1198-743X (Linking)
VI  - 14
IP  - 11
DP  - 2008 Nov
TI  - First direct detection of rickettsial pathogens and a new rickettsia, 'Candidatus
      Rickettsia barbariae', in ticks from Sardinia, Italy.
PG  - 1028-33
LID - 10.1111/j.1469-0691.2008.02082.x [doi]
AB  - The present study evaluated the molecular detection and identification of
      Rickettsia species in 83 ticks collected in Sardinia, Italy. Fifteen ticks were
      PCR-positive using gltA-specific and ompA-specific primers, leading to the
      identification of Rickettsia aeschlimannii in Hyalomma marginatum marginatum, R. 
      massiliae in Rhipicephalus turanicus and in Rhipicephalus sanguineus, and a new
      rickettsia, previously referred to as PoTiRb169 in Portugal, in four
      Rhipicephalus turanicus. This new species was further characterized by
      amplification and sequencing of three additional genes (ompB, sca4 and rrs).
      Using the current criteria to name a rickettsia, this uncultivated rickettsia can
      be given a Candidatus status, and we propose to call it 'Candidatus Rickettsia
      barbariae'. The detection of three tick-borne rickettsiae in Sardinia raises the 
      possibility that many cases of spotted fever considered by clinicians and health 
      authorities as Mediterranean spotted fever due to R. conorii could, in fact, be
      due to other rickettsiae, including those found in this study. Analysing skin
      biopsies of inoculation eschars in patients with spotted fever would be, together
      with continuing entomological surveys, the best way to increase our knowledge of 
      tick-borne rickettsioses in Sardinia and more generally in the Mediterranean
      basin.
FAU - Mura, A
AU  - Mura A
AD  - Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
FAU - Masala, G
AU  - Masala G
FAU - Tola, S
AU  - Tola S
FAU - Satta, G
AU  - Satta G
FAU - Fois, F
AU  - Fois F
FAU - Piras, P
AU  - Piras P
FAU - Rolain, J-M
AU  - Rolain JM
FAU - Raoult, D
AU  - Raoult D
FAU - Parola, P
AU  - Parola P
LA  - eng
SI  - GENBANK/EU272186
SI  - GENBANK/EU272187
SI  - GENBANK/EU272188
SI  - GENBANK/EU272189
PT  - Journal Article
PL  - England
TA  - Clin Microbiol Infect
JT  - Clinical microbiology and infection : the official publication of the European
      Society of Clinical Microbiology and Infectious Diseases
JID - 9516420
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - Humans
MH  - Italy
MH  - Ixodidae/*microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Rickettsia/*classification/genetics/*isolation & purification
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
EDAT- 2008/12/02 09:00
MHDA- 2009/01/03 09:00
CRDT- 2008/12/02 09:00
PHST- 2008/12/02 09:00 [pubmed]
PHST- 2009/01/03 09:00 [medline]
PHST- 2008/12/02 09:00 [entrez]
AID - S1198-743X(14)61813-9 [pii]
AID - 10.1111/j.1469-0691.2008.02082.x [doi]
PST - ppublish
SO  - Clin Microbiol Infect. 2008 Nov;14(11):1028-33. doi:
      10.1111/j.1469-0691.2008.02082.x.