Format

Send to

Choose Destination
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):253-7.

One-step purification procedure for UDP-N-acetylmuramyl-peptide murein precursors from Bacillus cereus.

Author information

1
Max-Planck-Institut für Entwicklungsbiologie, Abteilung II, Tübingen, F.R.G.

Abstract

A method is described for the rapid isolation of the activated murein precursors UDP-N-acetyl-muramyl-pentapeptide (UDP-MurNAc-pentapeptide) and UDP-MurNAc-tripeptide from Bacillus cereus. After accumulation of the precursors by inhibition of murein synthesis either in the presence of vancomycin (for the pentapeptide precursor) or D-cycloserine (for the tripeptide precursor) the cells were extracted with boiling water. Prior to high pressure liquid chromatography the material was freed from acid precipitable material. UDP-MurNAc-penta- and tripeptide were separated from other components by reversed-phase HPLC on Hypersil ODS using isocratic elution conditions with sodium phosphate buffer. The precursors were obtained with at least 98% purity and a yield of about 50 mumol from a 10-l culture of B. cereus.

PMID:
1904044
DOI:
10.1016/0378-1097(91)90166-8
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center