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J Cell Mol Med. 2009 Sep;13(9B):3141-50. doi: 10.1111/j.1582-4934.2008.00591.x. Epub 2008 Jun 28.

Analysis of molecular determinants of PRL-3.

Author information

1
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania.

Abstract

In order to analyse whether a C-terminal polybasic sequence represents a nuclear localization signal (NLS) we obtained several truncated and mutant forms of protein of regerating liver (PRL)-3 and evaluated their subcellular localization as compared to the wild-type form. Our results invalidate the hypothesis that this is an NLS. We also analysed the influence of the C- and N-terminal residues on the phosphatase activity of PRL-3. Our results provide in vitro evidence that the C-terminal CAAX motif, besides directing the protein farnesylation, plays an additional regulatory role by inhibiting the catalytic efficiency of PRL-3. Taking into account the results we obtained, as well as reported data, we propose a hypothetical molecular mechanism for the nucleocytoplasmic localization and transfer of PRL-3.

PMID:
19040419
PMCID:
PMC4516473
DOI:
10.1111/j.1582-4934.2008.00591.x
[Indexed for MEDLINE]
Free PMC Article

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