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Rev Salud Publica (Bogota). 2008 Mar-May;10(2):299-307.

Dengue-yellow fever sera cross-reactivity; challenges for diagnosis.

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1
Instituto de Virología, Universidad El Bosque, Bogota, Colombia.

Abstract

OBJECTIVE:

The Flavivirus genera share epitopes inducing cross-reactive antibodies leading to great difficulty in differentially diagnosing flaviviral infections. This work was aimed at evaluating the complexity of dengue and yellow fever serological differential diagnosis.

MATERIAL AND METHODS:

Dengue antibody capture ELISA and a yellow fever neutralisation test were carried out on 13 serum samples obtained from yellow fever patients, 20 acute serum samples from dengue patients and 19 voluntary serum samples pre- and post-vaccination with YF vaccine.

RESULTS:

Dengue ELISA revealed IgM reactivity in 46,2 % of yellow fever patients and 42 % of vaccinees. Sixteen out of 20 dengue patients (80 %) had high YF virus neutralisation titres.

CONCLUSIONS:

Such very high cross-reactivity data challenged differential laboratory diagnosis of dengue and yellow fever in areas where both flaviviruses co-circulate. New laboratory strategies are thus needed for improving the tests and providing a specific laboratory diagnosis. Cross-reactivity between Flaviviruses represents a great difficulty for epidemiological surveillance and preventing dengue, both of which demand urgent attention.

PMID:
19039426
[Indexed for MEDLINE]
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