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Virology. 2009 Feb 5;384(1):114-24. doi: 10.1016/j.virol.2008.10.035. Epub 2008 Nov 28.

SV40 lymphomagenesis in Syrian golden hamsters.

Author information

1
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA. amcnees@bcm.edu

Abstract

Simian virus 40 (SV40) isolates differ in oncogenic potential in Syrian golden hamsters following intraperitoneal inoculation. Here we describe the effect of intravenous exposure on tumor induction by SV40. Strains SVCPC (simple regulatory region) and VA45-54(2E) (complex regulatory region) were highly oncogenic following intravenous inoculation, producing a spectrum of tumor types. Three lymphoma cell lines were established; all expressed SV40 T-antigen, were immortalized for growth in culture, and were tumorigenic following transplantation in vivo. New monoclonal antibodies directed against hamster lymphocyte surface antigens are described. The cell lines expressed MHC class II and macrophage markers and were highly phagocytic, indicating a histiocytic origin. Many hamsters that remained tumor-free developed SV40 T-antigen antibodies, suggesting that viral replication occurred. This study shows that route of exposure influences the pathogenesis of SV40-mediated carcinogenesis, that SV40 strain VA45-54(2E) is lymphomagenic in hamsters, that hamster lymphoid cells of histiocytic origin can be transformed in vivo and established in culture, and that reagents to hamster leukocyte differentiation molecules are now available.

PMID:
19038412
PMCID:
PMC3648995
DOI:
10.1016/j.virol.2008.10.035
[Indexed for MEDLINE]
Free PMC Article

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