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Nat Methods. 2008 Dec;5(12):1005-10. doi: 10.1038/nmeth.1270.

A large genome center's improvements to the Illumina sequencing system.

Author information

1
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK.

Abstract

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

PMID:
19034268
PMCID:
PMC2610436
DOI:
10.1038/nmeth.1270
[Indexed for MEDLINE]
Free PMC Article

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