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Nat Struct Mol Biol. 2008 Dec;15(12):1272-7. doi: 10.1038/nsmb.1524. Epub 2008 Nov 23.

Structure of a RSC-nucleosome complex and insights into chromatin remodeling.

Author information

1
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Abstract

ATP-dependent chromatin-remodeling complexes, such as RSC, can reposition, evict or restructure nucleosomes. A structure of a RSC-nucleosome complex with a nucleosome determined by cryo-EM shows the nucleosome bound in a central RSC cavity. Extensive interaction of RSC with histones and DNA seems to destabilize the nucleosome and lead to an overall ATP-independent rearrangement of its structure. Nucleosomal DNA appears disordered and largely free to bulge out into solution as required for remodeling, but the structure of the RSC-nucleosome complex indicates that RSC is unlikely to displace the octamer from the nucleosome to which it is bound. Consideration of the RSC-nucleosome structure and published biochemical information suggests that ATP-dependent DNA translocation by RSC may result in the eviction of histone octamers from adjacent nucleosomes.

PMID:
19029894
PMCID:
PMC2659406
DOI:
10.1038/nsmb.1524
[Indexed for MEDLINE]
Free PMC Article

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