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Brief Funct Genomic Proteomic. 2008 Nov;7(6):454-9. doi: 10.1093/bfgp/eln046. Epub 2008 Nov 21.

Reverse genetics in zebrafish by TILLING.

Author information

1
HHMI and Division of Basic Science, Fred Hutchinson Cancer Research Center, B2-152, 1100 Fairview Ave. N., Seattle, WA 98109, USA. cmoens@fhcrc.org

Abstract

TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.

PMID:
19028802
PMCID:
PMC2899843
DOI:
10.1093/bfgp/eln046
[Indexed for MEDLINE]
Free PMC Article

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