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Lab Chip. 2008 Dec;8(12):2151-6. doi: 10.1039/b812443d. Epub 2008 Oct 23.

An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization.

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  • 1Department of Electrical and Computer Engineering, 2nd Floor ECERF, University of Alberta, Edmonton, CanadaT6G 2V4.


Fluorescence in situ hybridization (FISH) is a powerful technique for probing the genetic content of individual cells at the chromosomal scale. Conventional FISH techniques provide a sensitive diagnostic tool for the detection of chromosomal alterations on a cell-by-cell basis; however, the cost-per-test in terms of reagent and highly qualified labour has prevented its wide-spread utilization in clinical settings. Here, we address the inefficient use of labour with the first integrated and automated on-chip FISH implementation, one that requires only minutes of setup time from the technician. Our microfluidic chip has lowered the reagent use by 20-fold, decreased the labour time by 10-fold, and substantially reduced the amount of support equipment needed. We believe this cost-effective platform will make sensitive FISH techniques more accessible for routine clinical usage.

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