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Respiration. 2009;77(4):427-39. doi: 10.1159/000176209. Epub 2008 Nov 20.

Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes.

Author information

1
Laboratory of Clinical Microbiology and Virology, University Hospital Tor Vergata, Rome, Italy. marco.ciotti@ptvonline.it

Abstract

BACKGROUND:

Data have accumulated implicating the involvement of oncogenic human papillomaviruses (HPVs) in bronchial carcinogenesis. We recently described the presence of oncogenic HPV transcripts in non-small cell lung cancers.

OBJECTIVE:

To investigate the role of oncogenic HPVs in lung carcinogenesis.

MATERIAL AND METHODS:

The lung cell line A549 stably infected with HPV16E6, HPV16E7 and HPVE6/E7 constructs was used to investigate the protein profile changes associated with the expression of these oncogenes. Replicated two-dimensional gel electrophoresis gels from uninfected and stably HPV16E6-, E7-, and E6/E7-infected A549 cells were compared for changes in protein profile. Protein identification was achieved by peptide mass fingerprinting by MALDI-TOF-MS and nLC-ESI-Q-TOF-MS/MS peptide ladder sequencing.

RESULTS:

We identified 17 different polypeptides whose average normalized spot intensity was statistically significant (p < 0.05) and differed by 2-fold. Relationships between differentially expressed proteins and the HPV-induced infection mechanism have been clustered by knowledge-base database functional association network analysis.

CONCLUSION:

The impact of Hsp27, annexin III, annexin IV, Gp96 and TPT1 on the cellular response mechanism to HPV infection is presented and discussed.

PMID:
19023193
DOI:
10.1159/000176209
[Indexed for MEDLINE]

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