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Biosens Bioelectron. 2009 Jan 1;24(5):1405-10. doi: 10.1016/j.bios.2008.08.008. Epub 2008 Aug 13.

Nano-silver-modified PQC/DNA biosensor for detecting E. coli in environmental water.

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1
The Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China.

Abstract

To meet the requirement of World Health Organization for zero tolerance of E. coli cell in 100mL drinking water, a new procedure based on photodeposition of nano-Ag at TiO(2)-coated piezoelectric quartz crystal (PQC) electrode was developed to fabricate a highly sensitive PQC/DNA biosensor. Enhancement of 3.3 times for binding of complementary DNA has been shown and attributed to the following effects arising from the nano-Ag coating. First, a large increase in the active surface area and packing density of neutravidin enhances the maximum neutravidin load to 1.8 times of a normal electrode. Second, the functional activity of neutravidin is enhanced by chemical interaction with nano-Ag to give rise to an increase in the binding ratio between neutravidin and biotinylated DNA probe from 1.00:1.76 to 1.00:3.01. Third, the stronger binding leads to a higher stability of the biotinylated DNA probes bound and increase in hybridization with the complementary DNA. Under the optimized conditions for flow analysis with online PCR product denaturing and hybridization, a detection limit of eight E. coli cells are obtained which require sampling at least 800mL water to detect a single E. coli cell in 100mL water.

PMID:
19022649
DOI:
10.1016/j.bios.2008.08.008
[Indexed for MEDLINE]
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