Format

Send to

Choose Destination
See comment in PubMed Commons below
J Pharm Biomed Anal. 2009 Jan 15;49(1):55-63. doi: 10.1016/j.jpba.2008.10.002. Epub 2008 Oct 15.

Study of forced decomposition behavior of lamivudine using LC, LC-MS/TOF and MS(n).

Author information

1
Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar 160062, Punjab, India.

Abstract

Lamivudine was subjected to forced decomposition conditions of hydrolysis (neutral, acidic and alkaline), oxidation, photolysis and thermal stress, as suggested in the ICH guideline Q1A(R2). The drug showed instability in acid and alkali, while it remained stable in neutral conditions. It also degraded extensively under oxidative environment. It remained stable to light and thermal stress. In total, five degradation products were formed, which could be separated by LC on a C18 column using a gradient method. To characterize the products, first a complete fragmentation pathway of the drug was established by carrying out multi-stage (MS(n)) and MS/TOF accurate mass studies. The same was compared to fragment pattern of the degradation products resulting from LC-MS/TOF studies. The accurate mass values obtained from LC-MS/TOF were used to obtain elemental compositions, and the total information helped in identification of the degradation products. Subsequently, degradation pathway of the drug was laid down, along with mechanisms of formation of the degradation products. There is no previous information on these aspects on the drug in the literature.

PMID:
19022602
DOI:
10.1016/j.jpba.2008.10.002
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center