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J Biomed Opt. 2008 Sep-Oct;13(5):054049. doi: 10.1117/1.2983663.

Manipulation of mammalian cells using a single-fiber optical microbeam.

Author information

1
University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA. smohanty@uci.edu

Abstract

The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.

PMID:
19021429
PMCID:
PMC4034744
DOI:
10.1117/1.2983663
[Indexed for MEDLINE]
Free PMC Article
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