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Methods Mol Biol. 2009;485:41-53. doi: 10.1007/978-1-59745-170-3_4.

Cell-free assays for HIV-1 uncoating.

Author information

1
Department of Microbiology, Vanderbilt University School of Medicine, Nashville, TN, USA.

Abstract

Uncoating is an essential step in the retrovirus life cycle about which little is known. Uncoating is defined as the specific dissociation of the capsid shell from the viral core in the host cell cytoplasm. In this chapter, biochemical assays for studying HIV-1 uncoating in vitro are described. These techniques have proven useful for characterizing HIV-1 mutants that exhibit defects in the uncoating step of infection.

PMID:
19020817
PMCID:
PMC3842014
DOI:
10.1007/978-1-59745-170-3_4
[Indexed for MEDLINE]
Free PMC Article

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