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J Med Primatol. 2009 Feb;38(1):1-14. doi: 10.1111/j.1600-0684.2008.00299.x. Epub 2008 Nov 5.

MHC haplotype frequencies in a UK breeding colony of Mauritian cynomolgus macaques mirror those found in a distinct population from the same geographic origin.

Author information

1
Division of Retrovirology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, UK. emee@nibsc.ac.uk

Abstract

BACKGROUND:

Mauritian cynomolgus macaques have greatly restricted genetic diversity in the MHC region compared to other non-human primates; however, the frequency of common MHC haplotypes among captive-bred populations has not been reported.

METHODS:

Microsatellite PCR was used to determine MHC haplotype frequencies among captive macaques at a UK breeding facility. Allele-specific PCR and reference strand conformational analysis were used to determine the allele expression profile of a subset of animals.

RESULTS:

Haplotypes H3 (21%) and H1 (19%) were most common in the captive population of Mauritian cynomolgus macaques. Predicted alleles were detected by allele-specific PCR-SSP in 98% of animals. Allele expression profiles were similar in animals with identical haplotypes.

CONCLUSIONS:

Mauritian cynomolgus macaques in the UK breeding facility have restricted MHC diversity comparable to a previously described population. Microsatellite-derived haplotypes are highly predictive of allele expression. A selective breeding program has been established to produce MHC-identical animals for biomedical research.

PMID:
19018947
PMCID:
PMC4509677
DOI:
10.1111/j.1600-0684.2008.00299.x
[Indexed for MEDLINE]
Free PMC Article

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