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J Microsc. 2008 Nov;232(2):353-61. doi: 10.1111/j.1365-2818.2008.02106.x.

Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics.

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Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.


The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23 degrees C counterparts.

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