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Nat Rev Genet. 2009 Jan;10(1):57-63. doi: 10.1038/nrg2484.

RNA-Seq: a revolutionary tool for transcriptomics.

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1
Department of Molecular, Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, Connecticut 06520, USA.

Abstract

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

PMID:
19015660
PMCID:
PMC2949280
DOI:
10.1038/nrg2484
[Indexed for MEDLINE]
Free PMC Article
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