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J Chromatogr A. 2009 Apr 3;1216(14):2913-22. doi: 10.1016/j.chroma.2008.10.042. Epub 2008 Nov 1.

Analytical challenges in doping control: Comprehensive two-dimensional gas chromatography with time of flight mass spectrometry, a promising option.

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LAB DOP LADETEC/Instituto de Química, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, CEP 21941-909, RJ, Brazil.


Doping control screening based on the enhanced resolution of comprehensive two-dimensional (2D) gas chromatography hyphenated to time of flight mass spectrometer was investigated. The identification of anabolic agents (clenbuterol, norandrosterone, epimetendiol, two methyltestosterone metabolites and 3'-hydroxystanozolol) contained in a spiked urine sample (2ng/ml) was demonstrated. Special emphasis was given to 3'-hydroxystanozolol, mainly considering the difficulty in its detection. In contrast to conventional GC-MS approaches that must use single-ion monitoring, the GC x GC-TOFMS method enabled the identification of that metabolite through the deconvolution of the full mass spectrum and also resolved the co-eluted peaks of 3'-hydroxystanozolol and an endogenous component.

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