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Exp Cell Res. 2009 Jan 15;315(2):218-25. doi: 10.1016/j.yexcr.2008.10.021. Epub 2008 Oct 31.

Evidence for gamma-actin as a Z disc component in skeletal myofibers.

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Institute of Biomedicine, Department of Anatomy and Cell Biology, P.O. Box 5000 (Aapistie 7), FIN-90014 University of Oulu, Finland.


We investigated the targeting of the gamma-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged gamma-actin in rat flexor digitorum brevis myofibers. We found that the gamma-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific alpha-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the gamma-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of gamma-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells.

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