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Nat Methods. 2008 Dec;5(12):1027-30. doi: 10.1038/nmeth.1271. Epub 2008 Nov 16.

Nanoscale imaging of molecular positions and anisotropies.

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1
Department of Physics and Astronomy and Institute for Molecular Biophysics, 5709 Bennett Hall, University of Maine, Orono, Maine 04469, USA.

Abstract

Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

PMID:
19011626
PMCID:
PMC2901392
DOI:
10.1038/nmeth.1271
[Indexed for MEDLINE]
Free PMC Article
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