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Spine (Phila Pa 1976). 2008 Nov 15;33(24):2586-95. doi: 10.1097/BRS.0b013e3181883883.

The action of resveratrol, a phytoestrogen found in grapes, on the intervertebral disc.

Author information

1
Department of Biochemistry, Section of Rheumatology, Rush University Medical Center, Chicago, IL 60612, USA.

Abstract

STUDY DESIGN:

Basic science, biologic study.

OBJECTIVE:

To determine the potential benefits of using resveratrol (RSV) for intervertebral disc (IVD) matrix repair and regeneration.

SUMMARY OF BACKGROUND DATA:

The phytoestrogen RSV is a natural compound found in various plants including grapes and red wines. RSV has been reported to provide a protective effect on articular cartilage in rabbit models for arthritis, but its effect on spine cartilage is unknown. METHODS.: We studied the effect of RSV on bovine IVD cartilage homeostasis by assessing MMP-13 (potent catabolic factor) production, proteoglycan (PG) accumulation and synthesis, and the interaction between RSV and known catabolic factors such as bFGF or IL-1. To understand the molecular mechanisms by which RSV modulates MMP-13 and PG production, we also investigated its downstream target regulatory molecules.

RESULTS:

Stimulation of bovine disc cells cultured in monolayer with bFGF or IL-1 augmented the production of MMP-13 and ADAMTS-4 at the transcriptional level and this augmentation was blocked by RSV. Incubation of nucleus pulposus cells with RSV for 21 days significantly increased PG accumulation per cell in a dose-dependent manner, increased PG synthesis, rescued PG losses induced by catabolic reagents bFGF and IL-1, and promoted cell survival to levels seen after incubation with the anabolic protein BMP7 100 ng/mL. Protein-DNA interaction array results suggest that RSV effectively suppresses downstream target molecules of bFGF and IL-1 responsible for oxidative stress, proliferation, and apoptosis.

CONCLUSION:

Resveratrol is a potent anabolic mediator of bovine IVD cartilage homeostasis, revealing its potential as a unique biologic treatment to slow the progression of IVD degeneration. These data suggests RSV may have considerable promise in the treatment of disc degeneration.

PMID:
19011540
PMCID:
PMC2905225
DOI:
10.1097/BRS.0b013e3181883883
[Indexed for MEDLINE]
Free PMC Article

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