Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18290-5. doi: 10.1073/pnas.0809882105. Epub 2008 Nov 14.

Phage phi29 and Nf terminal protein-priming domain specifies the internal template nucleotide to initiate DNA replication.

Author information

1
Instituto de Biología Molecular "Eladio Viñuela," Centro de Biología Molecular "Severo Ochoa," Consejo Superior de Investigaciones Científicas-Universidad Autónoma C/Nicolás Cabrera 1, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.

Abstract

Bacteriophages phi29 and Nf from Bacillus subtilis start replication of their linear genome at both DNA ends by a protein-primed mechanism, by which the DNA polymerase, in a template-instructed reaction, adds 5'-dAMP to a molecule of terminal protein (TP) to form the initiation product TP-dAMP. Mutational analysis of the 3 terminal thymines of the Nf DNA end indicated that initiation of Nf DNA replication is directed by the third thymine on the template, the recovery of the 2 terminal nucleotides mainly occurring by a stepwise sliding-back mechanism. By using chimerical TPs, constructed by swapping the priming domain of the related phi29 and Nf proteins, we show that this domain is the main structural determinant that dictates the internal 3' nucleotide used as template during initiation.

PMID:
19011105
PMCID:
PMC2587616
DOI:
10.1073/pnas.0809882105
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center