Format

Send to

Choose Destination
Methods Mol Biol. 2009;478:71-92. doi: 10.1007/978-1-59745-379-0_4.

Biolistics transformation of wheat.

Author information

1
Centre for Crop Genetic Improvement, Department of Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, UK.

Abstract

We present a complete, step-by-step guide to the production of transformed wheat plants using a particle bombardment device to deliver plasmid DNA into immature embryos and the regeneration of transgenic plants via somatic embryogenesis. Currently, this is the most commonly used method for transforming wheat and it offers some advantages. However, it will be interesting to see whether this position is challenged as facile methods are developed for delivering DNA by Agrobacterium tumefaciens or by the production of transformants via a germ-line process (see other chapters in this book).

PMID:
19009439
DOI:
10.1007/978-1-59745-379-0_4
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center