Format

Send to

Choose Destination
Methods Mol Biol. 2009;510:145-63. doi: 10.1007/978-1-59745-394-3_11.

HCV replicons: overview and basic protocols.

Author information

1
University of Heidelberg, Heidelberg, Germany.

Abstract

Subgenomic replicons have been the first efficient cell-culture system for HCV and still are a valuable tool for studying different aspects of RNA replication. A variety of replicons based on different viral isolates and vector designs have been established. Here, I give a brief overview of viral isolates, applicable host-cell lines, replicon structures, and general considerations regarding replicon experiments, supplemented by basic protocols for in vitro transcription, electroporation, selection of replicon cells, transient replication assays, and northern hybridization.

PMID:
19009259
DOI:
10.1007/978-1-59745-394-3_11
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center