Format

Send to

Choose Destination
See comment in PubMed Commons below
Development. 2008 Dec;135(24):4101-11. doi: 10.1242/dev.027367. Epub 2008 Nov 12.

Canonical Wnt signaling is required for the maintenance of dorsal retinal identity.

Author information

1
Department of Neurobiology and Anatomy, University of Utah, 401 MREB, 20 N. 1900 E., Salt Lake City, UT 84132, USA.

Abstract

Accurate retinotectal axon pathfinding depends upon the correct establishment of dorsal-ventral retinal polarity. We show that dorsal retinal gene expression is regulated by Wnt signaling in the dorsal retinal pigment epithelium (RPE). We find that a Wnt reporter transgene and Wnt pathway components are expressed in the dorsal RPE beginning at 14-16 hours post-fertilization. In the absence of Wnt signaling, tbx5 and Bmp genes initiate normal dorsal retinal expression but are not maintained. The expression of these genes is rescued by the downstream activation of Wnt signaling, and tbx5 is rescued by Bmp signaling. Furthermore, activation of Wnt signaling cannot rescue tbx5 in the absence of Bmp signaling, suggesting that Wnt signaling maintains dorsal retinal gene expression by regulating Bmp signaling. We present a model in which dorsal RPE-derived Wnt activity maintains the expression of Bmp ligands in the dorsal retina, thus coordinating the patterning of these two ocular tissues.

PMID:
19004855
PMCID:
PMC2667153
DOI:
10.1242/dev.027367
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center