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Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):18064-9. doi: 10.1073/pnas.0808652105. Epub 2008 Nov 11.

Prefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis.

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1
Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA 94305, USA.

Abstract

Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.

PMID:
19004800
PMCID:
PMC2584705
DOI:
10.1073/pnas.0808652105
[Indexed for MEDLINE]
Free PMC Article
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