Format

Send to

Choose Destination
Gene. 1991 Jan 2;97(1):109-21.

Escherichia-Pseudomonas shuttle vectors derived from pUC18/19.

Author information

1
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences, Center, Canada.

Abstract

Two new broad-host-range plasmid vectors, pUCP18 and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.

PMID:
1899844
DOI:
10.1016/0378-1119(91)90016-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center