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Methods Mol Biol. 2008;491:141-9. doi: 10.1007/978-1-59745-526-8_11.

Whole-cell recording using the perforated patch clamp technique.

Author information

1
Institute of Membrane and Systems Biology, University of Leeds, UK.

Abstract

Many ion channels, particularly potassium channels, are regulated by intracellular substances, such as nucleotides or Ca(2+). These modulators are washed out of the cell during standard whole-cell patch clamp recordings, or maintained at a particular concentration if they are included in the pipette solution. Perforated patch clamp recording permits electrical access between the cell and the patch pipette using pore-forming antibiotics such as nystatin or amphotericin B. These are permeable to small monovalent ions but present a physical barrier to the larger impermeable ions and molecules. This maintains the integrity of many cytoplasmic components including soluble second messengers, and also helps to prevent channel "run down".

PMID:
18998090
DOI:
10.1007/978-1-59745-526-8_11
[Indexed for MEDLINE]

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