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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1074-8. doi: 10.1107/S1744309108033290. Epub 2008 Oct 31.

Crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 6.

Author information

1
Department of Biochemistry and Molecular Biology, School of Medicine, Oregon Health and Science University, Portland, OR 97239-3098, USA.

Abstract

Adeno-associated viruses are being developed as vectors for gene therapy and have been used in a number of clinical trials. Vectors to date have been based on the type species AAV-2, the structure of which was published in 2002. There is growing interest in modulating the cellular tropism and immune neutralization of AAV-2 with variants inspired by the properties of other serotypes. Towards the determination of a structure for AAV type 6, this paper reports the high-yield production, purification, crystallization and preliminary diffraction studies of infectious AAV-6 virions. The crystals diffracted to 3.2 A resolution using synchrotron radiation. The most promising crystal form belonged to space group R3 and appeared to be suitable for initial structure determination.

PMID:
18997346
PMCID:
PMC2581704
DOI:
10.1107/S1744309108033290
[Indexed for MEDLINE]
Free PMC Article

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