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Clin Biochem. 2009 Jan;42(1-2):114-7. doi: 10.1016/j.clinbiochem.2008.10.007. Epub 2008 Oct 25.

Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method.

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1
Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.

Abstract

OBJECTIVES:

To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL).

DESIGN AND METHODS:

The GP-HPLC and the ultracentrifugation (UC)/precipitation methods were used and compared.

RESULTS:

There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145+/-47 mg/L and 121+/-45 mg/L respectively, n=38, p<0.0001).

CONCLUSIONS:

A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.

[Indexed for MEDLINE]

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