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J Vis Exp. 2007;(10):574. doi: 10.3791/574. Epub 2007 Dec 13.

Derivation of human embryonic stem cells by immunosurgery.

Author information

1
Dept. of Molecular and Cellular Biology, Harvard University, USA. aechen@mcb.harvard.edu

Abstract

The ability of human embryonic stem cells to self-renew and differentiate into all cell types of the body suggests that they hold great promise for both medical applications and as a research tool for addressing fundamental questions in development and disease. Here, we provide a concise, step-by-step protocol for the derivation of human embryonic stem cells from embryos by immunosurgical isolation of the inner cell mass.

PMID:
18989406
PMCID:
PMC2557078
DOI:
10.3791/574
[Indexed for MEDLINE]
Free PMC Article
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